ABSTRACT
The genus Rabdosia is famous for the abundance of diverse and novel ent-kaurane diterpenoids. However, only a few ent-kauranoids have been discovered from R. flexicaulis since the investigation on its chemical constituents is not systematic. To find novel bioactive diterpenoids, the ethyl acetate extract of the above ground part of R. flexicaulis in Daofu County, Sichuan Province was obtained by column chromatography. One new compound and five known ones were identified as flexicaulin E(1), forrestin B(2), inf-lexarabdonin D(3), 7α-hydroxydehydroabietic acid(4), 15-hydroxydehydroabietic acid(5), and pomiferin F(6) by spectral techniques. Compounds 1-3 were the ent-kaurane diterpenoids isolated from this species for the first time. Compounds 4-6, aromatic abie-tanoids, were isolated from the genus Rabdosia for the first time.
Subject(s)
Diterpenes , Diterpenes, Kaurane , Isodon/chemistry , Molecular Structure , Plant Extracts/chemistryABSTRACT
<p><b>OBJECTIVE</b>To explore the effect of aluminum phosphate gel and Kangfuxin on esophageal pathology and expressions of interleukin-8 (IL-8) and prostaglandin E2 (PGE2) in rats with reflux esophagitis and explore the possible mechanisms.</p><p><b>METHODS</b>Sixty SD rats were randomized into aluminum phosphate gel group (n=10), Kangfuxin group (n=10), aluminum phosphate gel+Kangfuxin group (n=10), model group (n=20), and control group (n=10). Except for those in the control group, all the rats were subjected to infusion of diluted lysolecithin with hydrochloric acid in the esophagus for 14 days. Ten rats in the model group and those in the control group were sacrificed to examine the pathological changes and contents of IL-8 and PGE2 in the esophagus using optical and electron microscopes and radioimmunoassay. The next day the rest rats were given corresponding treatments (saline in model group) administered into the esophagus on a daily basis for 14 days, after which esophageal pathologies and IL-8 and PGE2 contents were examined.</p><p><b>RESULTS</b>The model rats showed obvious esophageal pathologies including inflammatory cell infiltration, vacuolar degeneration of the epithelial cells, esophageal erosion and even ulceration, with severe detachment of the epithelial cells. The rats in all the intervention groups showed lessened esophageal pathologies and lowered esophageal IL-8 and PGE2 contents compared with those in the model group. Esophageal mucosal injury index and IL-8 and PGE2 contents were all significantly lower in rats receiving combined treatment with aluminum phosphate and Kangfuxin than in those receiving either of the treatments (P<0.05).</p><p><b>CONCLUSIONS</b>Both Kangfuxin and aluminum phosphate gel are effective in the treatment for reflux esophagitis induced by lysolecithin and hydrochloric acid, and their therapeutic effects are achieved possibly by reducing IL-8 and PGE2 levels in the esophagus.</p>
Subject(s)
Animals , Rats , Aluminum Compounds , Pharmacology , Dinoprostone , Metabolism , Disease Models, Animal , Drugs, Chinese Herbal , Pharmacology , Esophagitis, Peptic , Drug Therapy , Metabolism , Esophagus , Pathology , Gels , Interleukin-8 , Metabolism , Phosphates , Pharmacology , Rats, Sprague-DawleyABSTRACT
<p><b>OBJECTIVE</b>To assess the correlation between familial clustering of hepatocellular carcinoma (HCC) and the level of anti-P53 in human serum in Guangxi.</p><p><b>METHODS</b>Enzyme-linked immunosorbent assay (ELISA) was used to detect anti-P53 in 164 members from 20 HCC families and 164 members from non-cancer control families. Univariate analysis was performed to assess the correlation between seral level of P53 antibody and familial clustering of HCC.</p><p><b>RESULTS</b>The level of P53 antibody was significantly higher in the members of HCC families than controls (Z=-3.04, P=0.002). After eliminating the interference of hepatitis B virus infection, this tendency still remains (P=0.011). And there was a significant difference between relatives of different degrees from HCC families (chi-square=11.593, P=0.021), with the expression of anti-P53 declining along with decrease in relationship coefficient. Furthermore, the number of individuals with high anti-P53 expression was also significantly greater in HCC families (95/164) than controls (71/164) (P=0.006). And the expression was rising along with the increasing HCC numbers (chi-square=16.068, P=0.000). Anti-P53 level was also greater in HCC families featuring sibling affection than parental affection (chi-square=12.679, P=0.002). Univariate analysis indicated that high expression of anti-P53 is a risk factor for development of HCC (OR=2.087, 95%CI: 1.270-3.431).</p><p><b>CONCLUSION</b>High level of anti-P53 expression may be a factor for the clustering of HCC families in Guangxi, China.</p>
Subject(s)
Adolescent , Adult , Child , Female , Humans , Male , Young Adult , Antibodies, Neoplasm , Blood , Genetics , Carcinoma, Hepatocellular , Blood , Genetics , Allergy and Immunology , China , Cluster Analysis , Family Health , Liver Neoplasms , Blood , Genetics , Allergy and Immunology , Risk Factors , Tumor Suppressor Protein p53 , Allergy and ImmunologyABSTRACT
To search for potential drugs with potent antitussive, expectorant, antiasthmatic activities and low toxicity, a series of verticinone-bile acids salts were prepared based on the clearly elucidated antitussive, expectorant and antiasthmatic activities of verticinone in bulbs of Fritillaria and different bile acids in Snake Bile. The antitussive, expectorant and antiasthmatic activities of these verticinone-bile acid salts were then screened with different animal models. Ver-CA (verticinone-cholic acid salt) and Ver-CDCA (verticinone-chenodeoxycholic acid salt) showed much more potent activities than other compounds. The bioactivities of Ver-CA and Ver-CDCA are worthy to be intensively studied, and it is also deserved to pay much attention to their much more potent antitussive effects than codeine phosphate. In order to elucidate whether they have synergistic effect and attenuated toxicity, their activities will be continuously compared with single verticinone, cholic acid and chenodeoxycholic acid at the same doses on different animal models. The application of "combination principles" in traditional Chinese medicinal formulations may be a novel way in triditional Chinese medicine research and discovery.
Subject(s)
Animals , Female , Male , Mice , Anti-Asthmatic Agents , Chemistry , Pharmacology , Antitussive Agents , Chemistry , Pharmacology , Asthma , Bile Acids and Salts , Chemistry , Pharmacology , Cevanes , Chemistry , Pharmacology , Chenodeoxycholic Acid , Chemistry , Pharmacology , Cholic Acid , Chemistry , Pharmacology , Cough , Drug Combinations , Drug Compounding , Methods , Drug Synergism , Expectorants , Chemistry , Pharmacology , Fritillaria , Chemistry , Guinea Pigs , Plants, Medicinal , Chemistry , Random Allocation , SnakesABSTRACT
<p><b>OBJECTIVE</b>To study the constituents from roots of Euphorbia hylonoma.</p><p><b>METHOD</b>Column chromatographic techniques were used for isolation and purification of the chemical constituents and their structures were identified by spectral analysis (IR, 1H-NMR, 13C-NMR, 2D-NMR and MS).</p><p><b>RESULT</b>Six compounds were isolated and elucidated as nonane (1), bis (2-ethylhexyl) phthalate (2), euphol (3), beta-sitosterol (4), acalyphol (5) and daucosterol (6) respectively.</p><p><b>CONCLUSION</b>Compounds 1, 2, 3, 5 and 6 were isolated from the plant for the first time.</p>
Subject(s)
Alkanes , Chemistry , Diethylhexyl Phthalate , Chemistry , Euphorbia , Chemistry , Lanosterol , Chemistry , Plant Roots , Chemistry , Plants, Medicinal , Chemistry , Sitosterols , Chemistry , Triterpenes , ChemistryABSTRACT
<p><b>OBJECTIVE</b>To study the chemical constituents from the rhizome of Impatiens pritzellii var. hupehensis.</p><p><b>METHOD</b>The rhizome were extracted with methanol, isolated and purified by column chromatography on silica gel. All the compounds were identified on the basis of spectral analysis (including IR, MS, NMR) and physico-chemical characters.</p><p><b>RESULT</b>Five compounds were identified as a-spinasterol (I), alpha-spinasteryl-7, 22-dien-3-O-beta-D-glucopyranoside (II), stigmast-7, 22-dien-3-one (III), stearic acid (IV), hentriantane (V).</p><p><b>CONCLUSION</b>Compound I is isolated from this plant for the first time, Compound II, III, IV, V are isolated from genus Balsaminaceae for the first time.</p>
Subject(s)
Impatiens , Chemistry , Plants, Medicinal , Chemistry , Rhizome , Chemistry , Stearic Acids , Chemistry , Stigmasterol , ChemistryABSTRACT
<p><b>AIM</b>To establish a fingerprint analysis method of Fritillaria hupehensis.</p><p><b>METHODS</b>fingerprint was performed by HPLC-ELSD. Hypersil ODS column was used; the mobile phase was composed of methanol (with 0.05% triethylamine) and water with gradient elution; flow rate was 1.0 mL x min(-1); recording time was 60 min; drift tube temperature was 75 degrees C; gas flow rate was 1.9 L x min(-1).</p><p><b>RESULTS</b>HPLC fingerprint of Fritillaria hupehensis was obtained.</p><p><b>CONCLUSION</b>A reliable method was provided for controlling the quality of Fritillaria hupehensis.</p>
Subject(s)
Alkaloids , Cevanes , Chromatography, High Pressure Liquid , Methods , Fritillaria , Chemistry , Light , Plant Roots , Chemistry , Plants, Medicinal , Chemistry , Quality Control , Reproducibility of Results , Scattering, Radiation , Sensitivity and SpecificityABSTRACT
<p><b>OBJECTIVE</b>To study the chemical constituents of hybridized Bulbus Fritillariae Ussuriensis.</p><p><b>METHOD</b>The chemical constituents were isolated by silica column chromatography and their structures were identified by physical and chemical eveidences and spectral analysis (IR, 1H-NMR, 13C-NMR, 2D-NMR, MS).</p><p><b>RESULT</b>Seven compounds were obtained and identified as (20S,25S)5alpha, 14alpha, 17beta-cevanine-6beta-hydroxy-3-one (hupehenirine, ZF1), (20S,25S)5alpha, 14alpha, 17beta-cevanine-3beta-hydroxy-6-one (hupehenizine, ZF2), (20R,25S)5alpha, 14alpha-cevanine-3beta,20beta-dihydroxy-6-one (peiminine, verticinone, ZF3), (20S,25S)5alpha, 14alpha, 17beta-cevanine-3beta, 6beta-dihydroxy (hupehenine, ZF4), (20R,25S)5alpha, 14alpha-cevanine-3beta, 6beta, 20beta-trihydroxy (isoverticine, ZF5), (20R,25S)5alpha, 14alpha-cevanine-3beta, 6alpha, 20beta-trihydroxy (peimine, verticine, ZF6), (20S,25S)5alpha, 14alpha, 17beta-evanine-6beta-hydroxy-3beta-O-beta-D-glucoside (hupeheninoside, ZF7).</p><p><b>CONCLUSION</b>Compounds ZF1-7 were isolated from hybridized Bulbus Fritillariae Ussuriensis for the first time.</p>
Subject(s)
Cevanes , Chemistry , Fritillaria , Chemistry , Classification , Genetics , Glucosides , Chemistry , Hybridization, Genetic , Molecular Structure , Plant Roots , Chemistry , Plants, Medicinal , Chemistry , Classification , GeneticsABSTRACT
<p><b>OBJECTIVE</b>To study the effect of different extracts of Alisma orientalis on urinary calcium oxalate stone formation in rats and to identify the effective constituents.</p><p><b>METHOD</b>Different extracts were administered through a stomach tube to rats of different groups with renal calcium oxalate stones induced by ethylene glycol (EG) and ammonium chloride (AC).</p><p><b>RESULT</b>In the rats administered with ethyl acetate elution of ethyl acetate extract, blood Cr, BUN, renal tissue calcium content, urinary calcium excretion and crystals deposition in renal tissue were significantly lower than those of the stone formation group.</p><p><b>CONCLUSION</b>The ethyl acetate elution of ethyl acetate fraction extract of Alisma orientalis can significantly inhibit urinary calcium oxalate stone formation in rats and be the most effective constituent of Alisma orientalis.</p>